Modulatory Effect of Ammonium Ions on Human Neutrophil Oxidative Burst in Response to Bacterial Stimuli

Neutrophils (polymorphonuclear leucocytes ) are the principal cells of the host defense system. Consequently, if periodontal pathogen – derived substances in the gingival crevice signifi- cantly inhibit their function, they could shift the bacterial – host balance in favour of the bacteria. The aim of investigation was to determine if the ammonium ions (periodontal pathogens produce substantial amounts ) affects the main neutrofils oxidative function – reactive oxygen species generation measured by luminol and lucigene– dependent chemiluminescence. Our results show that ammonium ions both in the group of non-periodontally diseased persons and in periodontitis patients have significant inhibitive effect on luminol- and lucigene-dependent chemiluminescence, which de- pends on NH4Cl concentration (5 mM, 10 mM, 25 mM, 50 mM), i.e. inhibitive effect increases with increasing concentration of NH4Cl . It shows that the presence of ammonia in the gingival crevice may increase the risk of development of periodontal disease.