Medicine by Alexandros G.Sfakianakis - Study on Th17,Treg cell and the expression of relative cytokines in psoriasis patients

Study on Th17,Treg cell and the expression of relative cytokines in psoriasis patients
Author(s): YANG Tingting, WANG Qing, LI Zhi, PIAO Jun, PIAO Jing'ai, Department of Clinical Laboratory, Dalian Municipal Central Hospital, Life Science College of Liaoning Normal University
Journal: Shanghai Journal of Medical Laboratory Sciences, Year 2015 , Issue 7 , Page 715-719
Keyword:  T helper cell 17; Regulatory T cell; Psoriasis;
Abstract: Objective To investigate the clinical significance of T helper cell 17( Th17),regulatory T( Treg) cell and the expression of relative cytokines in psoriasis patients. Methods Flow cytometry was used to analyze the percentages of Th17 and Treg cells in 28 patients with mild psoriasis,25 patients with severe psoriasis and 25 healthy controls. The mRNA expressions of retinoid-related orphan receptor gamma t( RORγt) and forkhead bos p3( Foxp3)were detected by reverse transcription polymerase chain reaction( PCR). The concentrations of interleukin 17( IL-17)and transforming growth facfor beta( TGF-β) in plasma of psoriasis patients were detected by enzyme-linked immunosorbent assay( ELISA). Results The percentages of Th17,RORγt mRNA and IL-17 in psoriasis patients increased compared with healthy controls,and there was a significantly higher levels in severe group than mild group( P < 0. 05). The percentage of Treg cells,Foxp3 mRNA and TGF-β in severe group were significantly lower than those in healthy controls and mild group( P < 0. 05). Conclusions The percentages of Th17 and Treg cells and their relative cytokines can be used as markers of psoriasis progression.
 
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